Abstract
Two methods are presented for simultaneous determination of diloxanide furoate and metronidazole. The first method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 242.5 and 285.5 nm for diloxanide furoate and 225.5 and 300 nm for metrinidazole. Calibration graphs were established for 2-100 I¼g mL-1 for diloxanide furoate and 1-50 I¼g mL-1 for metronidazole. In the second method (HPLC), a reversed-phase column with a mobile phase of ethylacetate:chloroform:dioxin:methanol (8:7:2:3 (v/v/v/v)) at 0.9 mL min-1 flow rate was used to separate both compounds with detection at 250-345 nm. Linearity was obtained in the concentration range of 0.025-50 I¼g mL-1 for diloxanide furoate and 0.15-50 I¼g mL-1 for metronidazole. All of the proposed methods have been extensively validated. These methods allow a number of cost and time saving benefits. The described methods can be readily utilized for analysis of pharmaceutical formulations. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The described HPLC method showed to be appropriate for simultaneous determination of diloxanide furoate and metronidazole in pharmaceutical dosage forms and in hyman serum samples.
Highlights
Diloxanide furoate is the drug of choice in the treatment of asymptomatic intestinal amoebiasis[1]
DD1 method: As shown in Fig. 1, it can be seen that the zero order absorption spectra of diloxanide furoate and metronidazole are very overlapped and as a result, the simultaneous determination of the two drugs cannot be possible for reliable direct absorbance measurements
To optimize the simultaneous determination of diloxanide furoate and metronidazole by using DD1 method, it is necessary to test the influence of the variables: divisor standard concentration, ∆λ and smoothing function
Summary
Diloxanide furoate is the drug of choice in the treatment of asymptomatic intestinal amoebiasis[1]. The most recent methods for determination of diloxanide furoate included chromatographic[2,3,4,5], electrochemical[69] and spectrophotometric[10,11,12,13] techniques. Mertonidazole has strong antiprotozoal and bactericidal actions[1]. Several methods have been reported for metrronidazole assay including chromatographic[14,15,16], electrochemical[17,18,19] and spectrophotometric[20,21,22,23] techniques. Diloxanide compounds with overlapping spectra[13,14,15]. The principles and advantages of this technique have been described by O’Haver and Green[24]
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