Abstract
Adrenaline, noradrenaline, dopamine and dihydroxyphenylalanine in brain tissue and dihydroxyphenylacetic acid in plasma have been determined by direct injection of brain homogenates or plasma into a liquid chromatographic system comprising three columns, one packed with a boronic acid gel and two with reversed-phase material. The catecholamines and dihydroxyphenylalanine were selectively adsorbed on the boronic acid gel and separated by ion-pair chromatography on the reversed-phase columns. DOPAC was determined in a modified system without the addition of ion-pairing reagents. The catechols were detected by coulometry or amperometry with two working electrodes, operated in the oxidative—reductive mode. The reductive signal was used for quantification. The limits of determination were 0.05 nmol g −1 with 40 mg brain tissue and 2 nmol 1 −1 with 0.5 ml plasma for DOPA and DOPAC respectively. The limit of quantification of DA and DOPAC in urine was about 0.1 μmol l −1.
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