Determination of diflubenzuron residues in milk and cattle tissues

S.A.V. Tfouni,R.M.S. Rocha,I.S.D. Loredo,L.A. Alves,R.P.Z. Furlani,A.M.D. Fonseca,A.G. Gomes,L.B. Carreiro,R.S.S. Mata

doi:10.1590/s0102-09352013000100043

Abstract

Diflubenzuron (DFB) is used to control ectoparasitic infestation by inhibiting larvae development in the manure and feces of treated animals. It is also currently been used to control tick infestations. In this study, milk and tissues from cattle treated orally with DFB for a 77-120 day period with a commercial product containing the compound were analyzed for the presence of residues. DFB residues were determined by using extraction with acetonitrile, cleanup with C18 SPE and chromatographic analysis by HPLC with UV detection (254nm). DFB was not detected in any of the analysed samples (<0.006mg kg-1 for fat, <0.014mg kg-1 for muscle, <0.015mg kg-1 for kidney, <0.016mg kg-1 for liver and <0.0006mg kg-1 for milk). In this manner, the use of this compound, according to the manufacturer's suggested doses may result in cattle milk, liver, kidneys, fat and muscles being considered safe regarding the presence of DFB residues.

Highlights

Diflubenzuron (1-(4-chlorophenyl)-3-(2,6-difluorobenzoyl) urea) is a benzoylurea insect growth regulator (IGR) used against larvae of different insects in agriculture and horticulture
Difluorobenzoyl) urea) is a benzoylurea insect growth regulator (IGR) used against larvae of different insects in agriculture and horticulture. It can be used in cattle breeding in order to prevent cattle tick (Boophilus microplus) and the development of horn fly (Haematobia irritans) infestations, since significant financial losses may result from ectoparasitic infestations such as these
Since there is a large consumption of meat and milk by the Brazilian population, it is important to assure that these products are safe regarding the presence of DFB residues

Summary

Introduction

Difluorobenzoyl) urea) is a benzoylurea insect growth regulator (IGR) used against larvae of different insects in agriculture and horticulture. It can be used in cattle breeding in order to prevent cattle tick (Boophilus microplus) and the development of horn fly (Haematobia irritans) infestations, since significant financial losses may result from ectoparasitic infestations such as these. The Diflubenzuron (DFB) mode of action involves the inhibition of the cuticle formation during insect development. It interferes with the synthesis of chitin, one of the components of the insect’s cuticle; the malformed cuticle. DFB was considered not fetotoxic or teratogenic and unlikely to be genotoxic or to pose carcinogenic risk to humans (JMPR, 2001)

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