Abstract
A Flow Injection Analysis (FIA) method for determination of molecular diffusion coefficients (Dm) of proteins, in a helically coiled tube, was reported. The coiling effect of the tube that causes secondary flow was avoided through using a Taylor-Aris and Golay's dispersion equation, modified by the velocity profile factor, K, and the modified equation was used for direct determination of diffusion coefficients. The experimental values of Dm were comparable with the literature values. The linear relationship between the Relative Molecular Mass (RMM, Mr) and the Molecular Diffusion Coefficient (Dm) was verified and a two-point calibration FIA method for estimation of Relative Molecular masses of proteins was suggested. Relative Molecular masses of two protein samples, hemoglobin and glucose oxidase were then estimated by this method. The values of RMM were 6.88x104 for hemoglobin and 16.0x104 for glucose oxidase.
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