Abstract
Dietary amino acid digestibility is a fundamental measure of importance in protein quality evaluation. Determining amino acid digestibility in humans, as the disappearance of an amino acid across the total digestive tract, has been discredited. Extensive cecal and colonic microbial metabolism renders fecal estimates of amino acids misleading. True ileal amino acid digestibility determined at the end of the small intestine predicts amino acid uptake more accurately. Given that ileal digestibility determination cannot be undertaken routinely in humans, a pig-based assay has been developed and validated. The growing pig values for digestibility, however, relate to healthy adult humans and there is a need to be able to determine amino acid digestibility for humans with specific physiological states. To this end, isotope-based methods for determining dietary amino acid digestibility indirectly show promise but remain to be fully validated.
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