Abstract
A reliable and sensitive competitive fluorescence immunoassay for the quantitative determination of dibutyl phthalate (DBP) was developed. The hapten of dibutyl phthalate was synthesized. The hapten‐protein conjugate was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production. With this polyclonal antibody, a novel fluorescence immunoassay for detection of DBP was described. Under best conditions, DBP can be determined in the concentration range of 0.1∼100 µg/L with a detection limit of 0.05 µg/L. The cross‐reactivities of the anti‐DBP antibody to six structurally related phthalate esters were below 9%. Some environmental samples were analyzed with satisfactory results.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
