Abstract

Abstract A sensitive method for the assay of diamine oxidase is described, based on the separation by reversed-phase high-performance liquid chromatography of the products arising from the oxidation of the reagents (A) homovanillic acid, (B) 3,5-dichloro-2-hydroxybenzenesulphonic acid—4-aminoantipyrine and (C) pyridoxine—4-amino-N,N-diethylaniline. The analysis time is short and the assay is quantitative and reproducible. Because of its speed and high sensitivity and selectivity, the method is a useful alternative to the standard spectrophotometric assay.

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