Abstract
A revised method for the determination of DHEA sulfate in human plasma is described. After extraction with methylene chloride as the pyridinium salt, DHEA sulfate is purified by thin-layer chromatography and hydrolyzed by solvolysis technique. The liberated DHEA is purified by the second thin-layer chromatography and determined by gas-liquid chromatography as the trimethylsilyl ether derivative. This method proved to be practical for processing large numbers of clinical samples and gave consistently reliable results.
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