Abstract
An enzyme sensor system was developed for the determination of dehydroascorbic acid and L-ascorbic acid. The system was constructed from a parallel flow cell separated by a dialysis membrane, an immobilized ascorbate oxidase membrane, coupled to a Clark-type oxygen electrode, and L-cysteine, which was present as a reductant. The method is based on the reduction of dehydroascorbic acid to L-ascorbic acid with L-cysteine in a parallel flow cell. One assay for dehydroascrobic acid could be completed within 8 min. Linear calibration curves for dehydroascorbic acid and L-ascorbic acid were observed in the range of 20 mM–100 mM and 2 mM–20 mM, respectively.
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