Abstract
Aim The method of D-limonene of Fructus Aurantii Immaturus volatile oil clathrates (clathrates) was established and the solubility of clathrates was determined. Methods: The detection method of clathrates was HPLC, and the solubility of clathrates was determined by equilibrium method. Results Welch UItimate XB-C18 chromatographic column (4.6 mm×250 mm, 5 μm); The detection wavelength: 205 nm; column temperature: 35℃; sample size: 10 μL; mobile phase: methyl cyanide (A)-water (B); current speed: 1 mL/min, gradient elution program: 0~10 min 65%~75% ( A); 10~28 min 75%~78% (A); 28~32 min 78%~65% (A); 32~42 min 65% (A). The solubility of the clathrates in water is 12.75 times that of the volatile oil. Conclusion HPLC method of D-limonene content and inclusion rate is accurate and reliable, it can be used as the method to measuring the rate of clathrates. And this experimental method is stable and reliable, can prepare the clathrates with good solubility and stable properties.
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