Abstract
Given the high importance of an accurate detection for quarantine plant pathogens, and the risk of false positive or false negative results, a detection method must be correctly validated. A statistical procedure has been developed to determine the cycle cut off and the corresponding limit of detection in real‐time PCR, considering a risk of false positive results of 1% and a probability of detection of 95%. The procedure presented in this paper is easy to develop and can be applied to any plant pathogen to be detected by real‐time PCR.
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