Determination of Curcumin in Turmeric Sample Using Edge Plane Pyrolytic Graphite Electrode

Abstract

Phenolic compounds are an important class of the antioxidants and found in several natural products. Research on the phenolic compounds having antioxidant properties present in natural pruducts like fruits, spices and herbs are increased in recent years. Electroanalytical methods have low detection limits very short analysis time and require less budge as well. These advantages make the electroanalytical methods favorable and voltammetric analysis methods are one of the preferred methods in determination of the compounds having antioxidant properties present in different matrices. In this study, curcumin in turmeric samples were quantitatively determined by using edge plane pyrolytic graphite electrode (EPPG) and differential pulse voltammetry. Electrooxidation behaviour of curcumin was also examined by using cyclic voltammetry method. A three electrode electrochemical cell was used for voltammetric analysis. Edge plane pyrolitic graphite (EPPG), was used as working electrode, saturated calomel electrode (SCE) was used as reference electrode and Pt wire electrode was used as counter electrode in the cyclic voltamety and differential pulse voltametry studies. A linear relationship between anodic peak current and curcumin concentration was observed between 0.325 μM to 1.95μM at EPPG electrode with differential pulse voltammetry. Detection limit was calculated as 0,296 μM and the method successfully applied for detection of curcumin amount in a turmeric sample.