Abstract

A method of quantitative determination of curcumin in Curcuma longa was established by using high performance liquid chromatography. Curcumin contents of methanol, aqueous methanol-(4 : 6) and water-extracts of turmeric (rhizomes of Curcuma longa L.) were determined by high performance liquid chromatography (HPLC) by use of Nucleosil C18 column as a stationary phase, a mixture of CH3CN : H2O : CH3COOH (51 : 49 : 5) as a mobile phase and benzyl benzoate as an internal standard. The present method is preferable to other analytical methods with regard to accuracy, simplicity and speed.

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