Abstract
In 27 isolates of Bacillus thuringiensis from southern provinces of Vietnam with ability to produce bipyramidal inclusions, 21 isolates were positive for cry2A-type genes via PCR methods. Analysis of DNA sequences revealed the amplified products belonged to cry2A-type genes of B. thuringiensis with identity above 90% compared with cry2A sequences published on GenBank sequence database. The identity values of the amplified products of the isolates BT5 and TN7.1 with cry2A group primers were 97% and 99%, respectively, while the identity of 99% were observed in the PCR products of TN7.1 with specific primers for partial sequences of cry2Aa and cry2Ab. These results may be useful for prediction of insecticidal activity of the B. thuringiensis isolates and development of new bioinsecticide products.
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