Abstract
Determination of Concentrations of Glucose and Human Serum Albumin in Mixtures in Phosphate-Buffered Solution by Near-Infrared Spectroscopy
Highlights
From a clinical viewpoint, blood substances can provide much valuable information about the physiological status of an individual
We attempted to observe positive peaks in the absorbance spectrum of human serum albumin (HSA) in phosphate-buffered solution (PBS) using differential spectra from a reference solution spectrum and found clear peaks corresponding to HSA concentration.[15]. We found that some peaks that could be observed in diffuse reflectance spectra of powder-state HSA could not be observed, and limited peaks in the powder state could be apparent in aqueous solution
Because such strong water absorption completely masks the absorption of glucose and HSA, it is very difficult to observe any features of dissolved glucose and HSA in the absolute absorption spectra
Summary
Blood substances can provide much valuable information about the physiological status of an individual. Current blood substance analyses mainly involve chemical and/or biological reactions with reagents, because of the high precision of such measurement, blood constituent measurement without any reagents, called “reagentless measurement”, has been studied in tandem with the advancement of measurement instrumentation and applied statistics.[1,2] Reagentless monitoring allows the possibility of measurement with rapid response, real-time monitoring, cost-effectiveness, and simultaneous measurement of multiple constituents with only one device.[1,2,3] there have been many attempts to realize this approach An example of such reagentless measurement, with a long history, is CO-oximetry, that is, the in vitro measurement of the oxygenation status of hemoglobin contained in red blood cells using visible light and near-infrared (NIR) radiation.[4,5] in vitro CO-oximetry has been expanded to the in vivo and noninvasive measurement technique named “pulse oximetry”, which aims to measure the oxygenation status of a patient’s hemoglobin transcutaneously.[6] Attempting to add to the success of these methods for hemoglobin oxygenation measurement, the development of reagentless techniques for blood constituents, other than those linked to oxygen, is still ongoing. The results that emerge could sometimes be unclear or inaccurate if there are artifacts with spurious correlation
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