Determination of Concentration of Zolmitriptan in Solid Dosage Form by UV Spectroscopic Method

Abstract

Zolmitriptan (ZMT) is a drug used in the treatment of migraines and cluster headaches. ZMT in bulk and pharmaceutical formulation determined by Zero order, First order derivative, Second order derivative and AUC by using UV-spectroscopic methods. The standard stock solution was prepared of 100 μg/ml for all proposed methods, and working standard solutions were prepared from the stock solution after adequate dilutions (1–10 μg/ml).For zero order derivative, standard solutions were measured at 283nm. For first order derivative spectroscopy the response (dA/dλ) of standard solutions was measured at 216 nm. Calibration curve was constructed by plotting response (dA/dλ) values against concentrations. Regression equation of linear calibration graph was calculated as (r2=0.994). The response for second order derivative spectroscopy was measured at 237nm, and the regression equation was calculated as (r2=0.992).The AUC-spectroscopic method was based on calculation of area under curve (AUC) for analysis of zolmitriptan in the wavelength range of278283nm, regression equation of linear calibration graph was calculated as (r2=0.993). All Developed methods were validated as per ICH guidelines. Hence the developed methods are used for routine analysis of solid dosage form containing Zolmitriptan.