Abstract

Two methods including essential oil extraction and preparation of herbal extract were used to determine the compositions of the Thymus pubescens (Boiss.et Kotschy ex Celak). The former was carried out though maceration by two solvents and the latter is done in a Clevenger apparatus using water. Gas chromatograph (GC) coupled to a mass spectrometer (MS) system was used to determine the composition of samples. The results showed that the major compositions of the aqueous extraction of essential oil are Alpha-pinene (0.9%), Borneol (10.36%), Thymol (28.01%), Trans-caryophylene (27.14%), delta-cadinene (4.30%), while for the ethanol extraction are alpha-pinene (10.71%), trans-caryophylene (73.42%), phenol (7.3%) and for acetone extraction are alpha-pinene (18.17%), cineole (11.23%), gamma-terpinene (7.26%), borneol (6.1%), thymol (9.41%), carvacrol (0.56%), and trans-caryophylene (29.62%).

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