Abstract
In this research, a new mode of dispersive liquid–liquid microextraction based on a double-solvent system (DLLME-DSS) was developed for the extraction and preconcentration of organophosphorus pesticides (OPPs) in the blood of children with acute leukaemia prior to determination by high-performance liquid chromatography-ultraviolet detection (HPLC-UV). In the present method, two long normal chain alcohols are mixed in a particular ratio, and then injected into the sample solution, which is magnetically stirred. In this case, the mixture of the two alcohols changes to a new aggregate extractant. This new double-solvent is used as an extractant, which has a higher extraction power than any of its components alone. Under the optimum conditions, the calibration graph was linear in the rage of 3–600 μg L−1 with detection limits of 1 to 2 μg L−1. Relative standard deviations (RSDs) including intra-day and inter-day of the method based on 7 replicate determinations of 100.0 μg L−1 for each analyte were in the range of 2.9–4.7% and 3.8–6.1%, respectively. The results proved that DLLME-DSS is a sensitive, very simple, inexpensive, environmentally friendly, rapid and efficient method for the preconcentration of trace amounts of OPPs in blood samples.
Highlights
Organopesticides are chemicals used to kill and control pests, diseases, weeds and microbes, and are most commonly used in agricultural products.[1]
The rst two long normal chain alcohols are mixed in a particular ratio, and it is injected into the sample solution, which is on the magnetic stirrer
Relative standard deviations (RSDs) including intra-day and inter-day of method based on 7 replicate determinations of 100.0 mg LÀ1 for each analyte were in the range of 2.9–4.7% and 3.8–6.1%, respectively
Summary
Analytical instrumentals such as high performance liquid chromatography (HPLC),[11,12,13] gas chromatography (GC)[14,15,16] and gas chromatography-mass spectrometry (LC-MS),[17,18,19] have been used for the determination of OPPs in different matrices. The sample preparation step is the most critical part of the analysis process, o en associated with spending much time, high organic solvents consumption, and inaccuracy To overcome these problems, miniature extraction techniques based on liquid–liquid extraction (LLE) have been developed today. A double-solvent system (DSS) was developed as an extractant without disperser solvent for DLLME In this method, the rst two long normal chain alcohols are mixed in a particular ratio, and it is injected into the sample solution, which is on the magnetic stirrer. The rst two long normal chain alcohols are mixed in a particular ratio, and it is injected into the sample solution, which is on the magnetic stirrer In this case, the mixture of the two alcohols is changed to new aggregate extractant and dispersed in tiny droplets with a very high contact surface in the sample solution. The DLLME-DSS was evaluated to determine the OPPs in blood of children with acute leukaemia
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