Abstract
This work presents a simple and fast method for colchicine (CO) determination in pharmaceutical formulations and urine by multiple pulse amperometry (MPA) with flow injection analysis (FIA) system using a boron-doped diamond electrode. In optimized conditions, it was possible to quantify CO in urine samples without interference of uric acid and ascorbic acid. The working linear range for CO quantification was achieved from 1.0 × 10-7 to 0.5 × 10-3mol L-1 with a low limit of detection of 2.14 × 10-8mol L-1. Furthermore, the proposed method showed high repeatability for 10 consecutive injections of 1.0 × 10-4mol L-1 CO (relative standard deviation = 1.28%) and good analytical frequency (30 determinations per hour). The addition and recovery studies in all pharmaceutical samples were approximately 100% and the results for CO determination were compared by high performance liquid chromatography (HPLC) with UV detection.
Highlights
Colchicine (CO) is an anti-inflammatory used in treatment of acute gouty arthritis
Thereby, this paper presents a simple and fast strategy for selective determination of CO in pharmaceutical formulations and urine samples using flow injection analysis (FIA)-multiple pulse amperometry (MPA) system and boron-doped diamond (BDD) as working electrode
The low relative standard deviation (RSD) value obtained by the proposed method can be justified by association of three factors: (i) BDD, a working electrode with a highly stable surface; (ii) FIA, a highly reproducible hydrodynamic system that allows a continuous cleaning of the working electrode during the analyses; and (iii) MPA detection, that improves the stability of the electrochemical signal due to the permanent application of cleaning pulses
Summary
Colchicine (CO) is an anti-inflammatory used in treatment of acute gouty arthritis. This drug has low therapeutic index, so the therapeutic effective concentration range is very narrow. Thereby, this paper presents a simple and fast strategy for selective determination of CO in pharmaceutical formulations and urine samples (recovery studies) using FIA-MPA system and BDD as working electrode.
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