Abstract
A non-aqueous reversed-phase liquid chromatographic method for determination of coenzyme Q 10 in pharmaceutical formulations has been developed. The reversed-phase system provides better reproducibility and better selectivity for the separation of coenzyme Q 10 analogues and degradation products than studied normal-phase systems. Furthermore, the non-aqueous mobile phase showed a very good solubility and provides a greater variety of work-up solvents for the lipophilic formulations than aqueous mobile phases. Validation studies showed detector response linearity over a concentration range of 0.2–100 μg/ml. The lower limit of detection was 2 ng on-column. The intra-assay precision (relative standard deviation) for a soy bean oil formulation was 2.0% ( n = 11).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
