Determination of Clopidogrel Bisulphate in Plavix Tablet and Human Biological Fluids Utilizing Chemically Modified Carbon Paste Sensor

Amal Fouad Khorshid

doi:10.4172/2155-9821.1000154

Abstract

The fabrication and the performance response characteristics of a novel sensitive, selective, simple, and rapid sensor for the determination of Clopidogrel bisulphate (CLO-H2SO4) were described. The sensing modified carbon paste sensor comprised of an ion-pair based on Clopidogrel with silico tungastate (CLO-ST) where this study included: composition, usable pH range, response time and temperature. The sensorex hibiteda wide linear dynamic concentration ranging from 1.00x10−7-1.00x10−2 and the usable pH ranges from 1.2-4.8 with the response time ranging from (5-8 sec) which is much faster compared to liquid ISEs with a detection limit equals 0.34 nM. The selectivity of the sensor (CLOH2SO4) was applied with respect to a many of organic and inorganic cations, amino acids and sugars. The application of the sensor (CLO-H2SO4) for its determination was utilized in bulk powder, Plavix Tablet, human (serum-urine) and monitoring Plavix tablet dissolution rates using calibration curve, standard addition, and the potentiometric titration methods. The obtained results were statistically analyzed in both accuracy and precision and were compared using the US pharmacopeia method where there is no significant difference was observed.

Highlights

Plavix is a trademark prescription medicine used to treat people who have any of the following: A heart attack, a Stroke or Recent Stroke, chest pain due to heart problems, poor circulation in their legs, acute Coronary Syndrome (ACS), established Peripheral Arterial Disease; use with Proton Pump Inhibitors (PPI), clopidogrel reduces the risk of heart attack and stroke in people who have cardiovascular disease [14]
No significant difference between two methods was observed with respect to accuracy and precision (Table 6)
The results prove the applicability of the three methods for the determination of CLO in the pharmaceutical formulation

Summary

Introduction

Plavix is a trademark prescription medicine used to treat people who have any of the following:. In spiked urine: For urine analysis, different quantities of the drug and 5 ml urine were transferred to a 100 ml volumetric flask and left stirred for 5min, completed to the mark with doubly distilled water and a small volume (0.1–2.0 ml) 0.01 M HCl was added to give solutions of pH ranging from 4 to 5 and concentrations from 1.0x10-6 to 5.0x10-4 M drug. These solutions were subjected to the standard addition method for drug determination.

Result and Discussion

Findings

Method

Conclusion