Abstract
A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of cis and trans-centchroman, an oral contraceptive, in bulk drug samples and its dosage forms. After extraction with methanol, separation was accomplished by reversed-phase HPLC on a C 18 column with acetonitrile—water (80:20) containing 0.4% of tetramethylammonium hydroxide [10% (v/v) aqueous solution] as the mobile phase. The pH was adjusted to 7.6 with 0.1 M orthophosphoric acid. The recoveries of cis- and trans-centchroman were always greater than 95%. The calibration graphs were linear over the range 0.11–4.0 μg for cis-centchroman and 0.18–4.0 μg for trans-centchroman.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
