Determination of chromium in human milk, serum and urine by electrothermal atomic absorption spectrometry without preliminary ashing

Abstract

In the present study a Perkin-Elmer 5000 atomic absorption spectrometer equipped with a tungsten—iodide lamp for improved background correction at the 357.9 nm chromium absorption line and an HGA 500 graphite furnace were employed for the direct determination of chromium in human serum, milk and urine. The method of standard additions was used: 0.25–0.75 ng Cr was added to 1 ml samples. Except for urine samples, a dilution of 1 + 1 to 1 + 2 with H 2O was necessary in order to obtain correct calibration curves. The average concentration of chromium in all the samples of normal subjects was less than 0.5 ng Cr ml −1. The day-to-day variation for all of the pooled samples was around 10% (relative standard deviation). For urine, the accuracy of the method was tested by comparing the results of another laboratory for the same two round robin samples. Excellent agreement was found between the present method and those of the other laboratory that had used isotope dilution—mass spectrometry and continuum source wavelength modulated echelle—atomic absorption spectrometry to define the chromium concentration in the samples. The detection limit of the method, 0.05 ng Cr ml −1 for urine and serum and 0.1 ng Cr ml −1 for human milk, was sufficient for the biological fluids analyzed. The method was employed for the determination of chromium in 24-h urine samples of maturity onset diabetics supplemented with 20 or 200 μg Cr 3+ d −1 for six weeks. It was shown that the 24-h urinary chromium excretion accurately indicates the daily dietary chromium intake of these patients.