Abstract

Benzoic acid, formed by the enzymatic hydrolysis of benzoylcholine with cholinesterase, is detected with a benzoate-sensitive membrane electrode made with tridodecylmethylammonium chloride as ion-exchanger and o-nitrophenyl octyl ether as plasticizer. The detection limits were 0.02 mM benzoate in phosphate buffer at pH 7.4 and 0.1 mM in the presence of blood serum. Cholinesterase in blood serum was determined successfully by using this electrode.

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