Determination of choline, putrescine and cadaverine in boletus by ion chromatography with suppressed conductivity detection

Abstract

A chromatographic method was developed for the determination of choline, putrescine and cadaverine in boletus by cation exchange chromatography with suppressed conductivity detection. Samples were extracted with 7 mmol/L methanesulfonic acid solution, then filtered through a membrane, and purified by a reversed phase solid phase extraction column. Choline, putrescine, cadaverine and coexisting ions in the samples were separated well on the IonPac CS17 cation exchange column (250 mm×4 mm). The mobile phase was 7 mmol/L methanesulfonic acid solution. All analytes could be eluted from the column in 25 min. The limits of detection (S/N=3) of choline, putrescine and cadaverine were 0.002, 0.002 and 0.003 mg/L, respectively. Moreover, they all had wide linearity ranges (0.01-10 mg/L) and satisfactory recoveries (91.6%-100.2%). This method is suitable for the determination of choline, putrescine and cadaverine in boletus, and has good separation performance, high selectivity and sensitivity.