Determination of choline containing phospholipids in serum, bile and amniotic fluids by the derivative enzymatic–spectrophotometric method

Abstract

A comparison was performed of the analytical results obtained by applying a well-known enzymatic–spectrophotometric method for the determination of choline containing phospholipids in biological human fluids (sera, bile and amniotic fluids) following the standard procedure method, or else using the first or second derivative methods. In terms of result reproducibility the comparison was extended to include also a biosensor-based method developed in recent years by the present authors. Some advantages are associated with using the first derivative method in the case of serum samples containing blood traces, especially via the ‘Gran’s plot’ method.