Determination of cholesterol in sera

Abstract

Two methods are described for the determination of cholesterol in sera. The principle of these methods is that hydrogen peroxide, liberated by action of cholesterol esterase and cholesterol oxidase on cholesterol esters (or free cholesterol), is coupled under the catalytic effect of peroxidase with 4-hydroxyphenylacetic acid to form a fluorescent product, and with 3-methylbenzothiazoline-2-one hydrazone and 3-dimethylaminobenzoic acid to obtain a coloured compound. The main advantages of these procedures over comparable methods are their short reaction time, high sensitivity and low cost of the tests.