Abstract
A new method for the determination of cholesterol in blood plasma or serum is described in which cholesterol esters are hydrolyzed with benzyltrimethylammonium hydroxide during evaporation of the alcohol-ether extract and the residue is then used directly for the Liebermann-Burchard reaction. Data are presented to show that from 89 to 104% of the cholesterol added to alcohol-ether extracts of plasma can be recovered by this method, and that there is good agreement with values determined by the method of Schoenheimer and Sperry.
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