Determination of cholesterol in blood. Part 3

Abstract

The chronological development of procedures for determining the concentration of cholesterol in plasma, serum, and whole blood is presented in the review. It is stated that, since the correlation between the risk of development of cardiovascular diseases and the concentrations of total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in human blood has been established by numerous medical studies, procedures for the measurement of these parameters have been developed most actively. A brief overview of these procedures and the results of their comparative tests in medical examinations of patients are given. Classifications are also proposed for procedures that determining total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in blood. The mechanism of action of the chemical reactions taking place in these procedures, the advantages and disadvantages, and prioritization of the field of their application are considered. Promising directions in the development and improvement of procedures are mentioned, ensuring more accurate measurements of the blood cholesterol concentration, and alternative means of determining cardiovascular disease risk are discussed.