Abstract
We developed a capillary zone electrophoresis (CZE) method with indirect UV detection for the simultaneous determination of major constituents, such as chloride (expressed as Cl−), inorganic phosphate (PO43−), and citric acid in a human serum. The limits of detection (LODs) for Cl−, PO43−, and citric acid were, respectively, 4.7, 2.0, and 1.3 μmol/L at a signal-to-noise ratio of three. The relative standard deviation (RSD, n = 8) of the peak area obtained for Cl−, PO43−, and citric acid in human serum from a healthy adult without deproteinization were 1.0, 1.6, and 4.0%, respectively. The analytes were detected within 18 min. Dproteinization was not required using a background electrolyte (BGE) containing 0.01% hydroxypropylmethylcellulose (HPMC). The analytical results for Cl− and PO43− in serum samples for quality control and human serum samples from healthy adults obtained by the proposed method agreed with those obtained by conventional methods. The proposed procedure could be useful as a simple diagnostic method for patients suffering from some diseases.
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