Abstract

Abstract The results of a comparative study of chloramphenicol (CAP) determination in meat, by high performance liquid chromatography are presented. A spectrophotometrical detector at 278 nm was used for measurement of CAP absorbance and subsequent quantitative determination. Two different mobile phases, were tested. The first phase consisted of acetonitrile—water (30:70 v/v) and the second of acetonitrile—di-ammonium hydrogen phosphate 0.005M (25:75 v/v). The two procedures were compared by means of the retention time and the sensitivity achieved. Recoveries of CAP from meat samples ranged between 63–79%. The detection limits for the two procedures were 14.1 μgμwkg−1 and 18.0 μgμkg−1 respectively.

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