Abstract
The R-(−)- and S-(+)-enantiomers of ethyl nipecotate tartaric acid salt were separated by chiral high performance liquid chromatography on a commercially available chiral stationary phase using a non-polar mobile phase. Samples of ethyl nipecotate tartaric acid salt were analysed on a Chiralcel-OG column as the free base of ethyl nipecotate after extraction. The mobile phase was hexane-2-propanol-2-methyl-2-propanol (94:4:2, v/v/v), to which ca. 0.5 ml/l of dimethylamine was added. The method is able to separate the two enantiomers with a resolution factor ( R s) of approximately 1.3 and a selectivity factor (α) of 1.15. The limit of quantification of the S-(+)-enantiomer is 0.2% in the R-(−)-enantiomer. The method was validated by the standard addition method and determining the recovery of the S-(+)-enantiomer in the R-(−)-enantiomer. The precision of the method was determined by analysing seven individual sample preparations. The analysis was done by two analysts on different days, using different equipment and reagents.
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