Abstract
Serum levels of cell-free interleukin-2 receptors were elevated above normal in mice bearing the IL-2R positive T-cell lymphoma Eb or its highly metastatic variant ESb. Although ESb cells expressed less IL-2R molecules than Eb cells on their cell surface, serum receptor levels were raised more quickly in ESb than in Eb tumour bearing animals. Elevated IL-2R serum levels were a sensitive tumour marker in animals bearing the aggressive variant ESb but not in animals bearing the low metastatic line Eb. Peritoneal ascites tumour-bearing animals had higher serum IL-2R levels than corresponding animals with subcutaneously growing tumours. Thus, serum IL-2R levels in tumour-bearing animals were dependent on the tumour line and influenced by the site and mode of tumour growth.
Highlights
Eously while the spontaneous in vivo variant ESb is. of high metastatic capacity in the spontaneous metastasis assay (Schirrmacher et al, 1982)
The low metastatic parental line Eb was found to express more IL-2 receptors as detected both by binding of the monoclonal anti IL-2R antibody AMT-13 (Osawa & Diamantstein, 1984a,b) and by absorption of IL-2 activity
We will demonstrate that soluble IL-2R can be detected in the serum of normal and tumour bearing animals when using a sensitive enzymelinked immunosorbent assay with two monoclonal antibodies that recognize distinct epitopes on the mouse IL-2 receptor
Summary
Eously while the spontaneous in vivo variant ESb is. of high metastatic capacity in the spontaneous metastasis assay (Schirrmacher et al, 1982). Of high metastatic capacity in the spontaneous metastasis assay (Schirrmacher et al, 1982). A plastic adherent variant, ESb-M, was isolated in tissue culture from the high metastatic line ESb and was shown to have greatly decreased metastatic capacity in vivo (Fogel et al, 1983). The low metastatic parental line Eb was found to express more IL-2 receptors as detected both by binding of the monoclonal anti IL-2R antibody AMT-13 (Osawa & Diamantstein, 1984a,b) and by absorption of IL-2 activity. We will demonstrate that soluble IL-2R can be detected in the serum of normal and tumour bearing animals when using a sensitive enzymelinked immunosorbent assay with two monoclonal antibodies that recognize distinct epitopes on the mouse IL-2 receptor. We will describe and discuss differences observed with cell lines of high and low metastatic capacity
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