Abstract
A high performance liquid chromatographic method for the quantitation of celecoxib (CEL) in human plasma is presented. The method is based on liquid–liquid extraction with chloroform and reversed-phase chromatography using a Nucleosil CN column ( 250 mm×4.6 mm i.d., 5 μm particle size) and UV spectrophotometer detection at 260 nm. The mobile phase consists of acetonitrile:water (60:40 (v/v)). Flutamide was used as internal standard (IS). The assay was linear in the concentration range of 10–1000 ng/ml when 0.5 ml aliquots of plasma were extracted. Within-day and between-day precision expressed by relative standard deviation is less than 4% and inaccuracy does not exceed 3%. The assay was used to analyze samples collected during human clinical studies.
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