Determination of Cefuroxime Axetil and Cefixime Trihydrate in Pharmaceutical Dosage Forms by RP-HPLC Method

Bashir Elias,Mohammad Anas Alfeen

doi:10.4172/2471-2698.1000114

Abstract

Determination of two generation Cephalosporins (Cefuroxime Axetil and Cefixime Trihydrate) in pharmaceutical dosage forms was carried out employing High Perfeormance Liquid Chromatographic using isocratic separation. Separation was performed on an Enable C18 column (250 mm × 4.6 mm, 5.0 μm) using Triethylamine: Methanol: Acetonitrile: Ultra-Pure Water (2:10:20:68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265 nm. The linearity was observed over a concentration range of 0.1-80 μg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were 99%. The developed method was used successfully for the determination of Cefuroxime Axetil, Cefixime Trihydrate, in Capsule, Tablet and dry syrup formulations.

Highlights

Cefuroxime Axetil (CA): 1-Hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxylamido]-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylate (Figure 1) is a second generation cephalosporin antibiotic [1].Cefixime Trihydrate (CT): (6R,7R)-7-{[(2R)-2-amino-2phenylacetyl]amino}-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylic acid hydrate (Figure 2) is a third generation cephalosporin antibiotic [1].It is used in the treatment of susceptible organsims of the respiratory tract, urinary tract, and skin [2]
Specificity: The specificity of the HPLC method was determined by checking the interference of any of the possible degradation products and Absorbances produced during study of CA and CT
Optimization of the mobile phase was carried out based on the tailing factor and theoretical plates obtained for CA and CT

Summary

Introduction

Cefuroxime Axetil (CA): 1-Hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxylamido]-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylate (Figure 1) is a second generation cephalosporin antibiotic [1].Cefixime Trihydrate (CT): (6R,7R)-7-{[(2R)-2-amino-2phenylacetyl]amino}-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylic acid hydrate (Figure 2) is a third generation cephalosporin antibiotic [1].It is used in the treatment of susceptible organsims of the respiratory tract, urinary tract, and skin [2]. Cefuroxime Axetil (CA): 1-Hydroxyethyl (6R,7R)-7-[2-(2-furyl) glyoxylamido]-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylate (Figure 1) is a second generation cephalosporin antibiotic [1]. Cefixime Trihydrate (CT): (6R,7R)-7-{[(2R)-2-amino-2phenylacetyl]amino}-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylic acid hydrate (Figure 2) is a third generation cephalosporin antibiotic [1]. It is used in the treatment of susceptible organsims of the respiratory tract, urinary tract, and skin [2]. There are different analytical methods reported for the determination of CA H3C O CH3 OO N N H S HH

Methods

Results

Conclusion