Abstract
A simple and fairly fast capillary zone electrophoresis method has been developed for the determination and separation of ceftriaxone, ceftizoxime, paracetamol, and diclofenac sodium in a mixture, in pharmaceutical formulations, and in blood serum. A 50 mM sodium tetraborate background electrolyte solution (pH 9.0) was found to be suitable for separation of all the drugs. An uncoated fused-silica capillary of a total length of 57 cm (effective length 50 cm) was used for separation. All the analytes were completely separated within 8.0 min at an applied voltage of 30 kV and detection was performed at 214 nm .Validation of the method was performed in terms of linearity, accuracy, precision and limit of detection, and quantification. The linearity of the calibration curves for paracetamol was 5-125 \mu g/mL, for ceftizoxime was 5-500 \mu g/mL, for diclofenac was 1-125 \mu g/mL, and for ceftriaxone was 10-1000 \mu g/mL, while LOD of the paracetamol, ceftizoxime, diclofenac, and ceftriaxone was found to be 1.0, 1.0, 0.5, and 5.0 \mu g/mL, respectively. The proposed method was applied for the determination of active ingredients in pharmaceutical formulations and active drugs in human blood serum. The recovery was found to be >= 99% with the relative standard deviation (RSD) Keywords: Capillary zone electrophoresis, paracetamol, ceftriaxone, ceftizoxime, diclofenac sodium
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
