Abstract
Chemically amplified biosensors based on substrate recycling using oxidase and reducing agents have been applied to the convenient detection of catecholamines and uric acid in biological fluids. These methods are based on the highly sensitive detection of trace amounts of analytes (10 −8 M level) in highly diluted (1000–10000 fold) body samples, in which the interfering species are also diluted to negligible concentration level for an amperometric measurement. The concentration of uric acid obtained by the chemically amplified uric acid sensor is in fairly good agreement with that obtained by conventional spectrophotometry.
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