Abstract
A biosensor was fabricated by incorporating laccase in a ZnO sol-gel with chitosan as a matrix for the determination of catechol. The ZnO nanoparticles were characterized by X-ray diffraction and atomic force microscopy. The conductivity of the chitosan/ZnO/glassy carbon electrode film was investigated by alternating current impedance. The biosensor was employed to monitor the reduction of catechol, and the peak current increased linearly with concentration between 1.0 × 10−6 and 1.0 × 10−4 mole per liter with a limit of detection of 2.9 × 10−7 mole per liter. The laccase biosensor exhibited good stability, reproducibility, and some selectivity.
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