Abstract
A simple and rapid ion-exchange chromatographic method with conductimetric detection for the determination of the main carboxylic acids (acetic, lactic, succinic, malic, citric and tartaric acids) and inorganic anions (chloride, nitrate and sulphate) in wines is described. Separation was optimized using the modified simplex method. The mobile phase is 0.975 m M phthalic acid at pH 4.15. The chromatographic system is kept at 39°C. The method does not require derivatization or extraction of the sample. The procedure only includes filtration (0.45 μm) and passage through a Sep-Pak C 18 cartridge. The linearity, sensitivity, recovery (>98%) and reproducibility (>99%) were studied for each acid. Detection limits ranging from 4.7 to 0.31 mg/l were obtained for citric acid and chloride, respectively. Comparison of the results with those obtained by spectrophotometric, GC, potentiometric and enzymatic assays showed that the values were similar. The ion-exchange chromatographic-conductimetric detection method permits the analysis of sweet wines without interference from sugars, and simplifies a type of determination which by other methods is complex or inaccurate.
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