Abstract
A technique for the estimation of carbonic anhydrase (CA) using the infra-red-gas analyser (IRGA) has been developed. Instead of determining pressure changes in the dehydration reaction catalysed by the enzyme, the IRGA was used to determine the increase in carbon dioxide content in the enzymatic and non-enzymatically catalysed dehydration reaction. The reliability of this method was assessed using pure carbonic anhydrase. Although the method has not been compared with other methods, the known sensitivity of the IRGA (resolution of 0.1–1.0 μmol/mol) was exploited. The differences in CA activity of two finger millet ( Eleusine coracana) genotypes differing markedly in photosynthetic rate but showing similar leaf conductances was assessed.
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