Abstract
A potentiometric method capable of determining carbonic anhydrase (CA) activity in vitro and based on the use of a pCO 2 sensor is presented. By means of the procedure described here it is possible to follow the rate of CO 2 diffusion that takes place in a buffered solution of NaHCO 3 in either the presence or the absence of CA. All experimental parameters that affect the speed of HCO 3 − dehydration, as well as the speed of CO 2 diffusion, can be fixed and kept constant for the duration of every assay. The advantage of this method is that the overall dehydration plus diffusion process can be followed as it actually takes place in open thermodynamic systems far from equilibrium. The results obtained strongly confirm the hypothesis of a facilitating role of CA toward the rate of CO 2 diffusion.
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