Determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-high resolution mass spectrometry coupled with dispersive micro solid-phase extraction

Abstract

A rapid method was established for the determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-Q Orbitrap high resolution mass spectrometry and dispersive micro solid-phase extraction (DMSPE) based on a strong cation exchange adsorbent (PCX). For the pretreatment method, the amount of PCX, the volume percentage of acetonitrile, the volume percentage of ammonium hydroxide and the volume of eluent were optimized. The analytes were separated on a BEH C18 column (50 mm×2.1 mm, 1.7 μm) and detected using targeted single ion monitoring-data dependent tandem mass spectrometry (tSIM-ddMS2) scan mode. The method showed a good linearity within a certain concentration ranges with correlation coefficients R2 ≥ 0.9999. The detection limits of carbendazim and thiabendazole in wine and beer were 0.02 and 0.01 μg/L, and the quantification limits were 0.06 and 0.03 μg/L. The average recoveries of carbendazim and thiabendazole at the spiked levels of 0.1, 1.0 and 100 μg/L were in the ranges of 95.6%-110.2% and 87.5%-102.8%, with intra-day precision (RSDr) ranges from 1.8%-5.2% and 1.3%-4.8%, and inter-day precision (RSDR) ranges from 4.3%-8.7% and 4.8%-9.4%. The method is rapid, simple and sensitive for the simultaneous determination of carbendazim and thiabendazole in wine and beer.