Abstract
Camelina oil has a high sterol concentration and is rather expensive compared to other vegetable oils. Because of its higher price, it is often adulterated by the addition of other, cheaper oils. This study was performed to validate a method for sterol determination in camelina oil, enabling the detection of camelina oil adulteration. Sterol levels in camelina oil samples were determined by gas chromatography after saponification and solid phase extraction. The method was validated, and the results proved that the chosen method is specific and selective, repeatable and accurate. The quantitatively assessed average contents of sterols in camelina oil samples of Slovenian origin were 21.4 mg 100 g-1 for brassicasterol, 153.6 mg 100 g-1 for campesterol, 3.9 mg 100 g-1 for stigmasterol, and 447.0 mg 100 g-1 for b-sitosterol. Results of camelina oil authenticity studies regarding botanical origin, performed by Principal Component Analysis (PCA) and Regularized Discriminant Analysis (RDA) enabled us to differentiate 100 % camelina oils from camelina oils adulterated with 10 %-40 % added sunflower, rapeseed or soya oil.
Highlights
In Slovenia, the production of camelina (Camelina sativa (L.) Crantz) is maintained as an alternative oilseed crop with nutritionally important value in seed or oil form
Chromatographic peaks were identified on the basis of retention time in comparison with the standards brassicasterol, campesterol, stigmasterol, and β-sitosterol, and the internal standard betulin
The results show that the method is specific and selective since there were no overlapping peaks
Summary
In Slovenia, the production of camelina (Camelina sativa (L.) Crantz) is maintained as an alternative oilseed crop with nutritionally important value in seed or oil form. Camelina oil is produced by crushing and warm-pressing the seeds, it is very susceptible to oxidation that can lead to oil quality loss.[3,4,5]
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