Abstract
A simple and rapid method was developed for the determination of calcium pantothenate(Pa-Ca) or panthenol(Pol) in pharmaceutical preparations by high performance liquid chromatography(HPLC). They could successfully be separated on a column of Nucleosil 5C18 with a mixture of 10 mM phosphate buffer (pH 3.0)-acetonitrile (97 : 3) as a mobile phase and detected at 208 nm. Aqueous extract from a preparation was applied to a Chemco-Sep ODS-A pretreatment column, and Pa-Ca and Pol were eluted with the above mobile phase. The eluate was made up to a constant volume and injected to HPLC system. The contents of Pa-Ca and Pol in the preparation were calculated by using calibration curves based on their peak heights on HPLC chromatogram. Several vitamin preparations were analyzed by the proposed method.
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