Abstract
Antiserum directed against calcium binding protein (CaBP) isolated from human kidneys was used for the immunofluorescent localization of CaBP in human intestine. Frozen sections of intestine obtained by biopsy from normal persons were tested by the indirect fluorescent antibody technique. Specific fluorescence indicating the presence of CaBP was observed at both the basal and apical poles of the intestinal absorptive cells while the goblet cells appeared to fluoresce non-specifically. Treatment of rachitic children with 25-hydroxyholecalciferol generally restored the pattern of fluorescence seen in intestinal tissue from normal persons. Concentration of CaBP as determined by the LAUREL-technique increades after treatment.
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