Abstract
At present, the commonly used HPLC method for the analysis of caffeine content in tea brews employs direct application of the samples on the column. This practice gradually reduces the efficiency of the column and shortens its life. In the modified method, the interfering tea pigments are effectively removed by passing the sample through a Sep-Pak C18 cartridge. Then its injection on a reversed phase μ-Bondapak C18 column employing acetonitrile and water (20:80 v/v) as mobile phase reduces the analysis time without affecting either the resolution of the peaks or the accuracy of caffeine determination. This method is shown to estimate accurately soluble caffeine contents in the brews of black tea, decaffeinated tea, and decaffeinated instant tea samples. Thus, the method is ideally suited for rapid routine anaylsis of black tea and its products. Keywords: Caffeine; Sep-Pak C18; decaffeinated tea; HPLC; instant tea
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