Abstract
A gas chromatographic method has been developed for the rapid, precise, and specific determination of caffeine in plasma. The method overcomes the major drawbacks of previous methods for the determination of caffeine in body fluids, which were: difficult isolation from interfering materials, a substantial blank error and low sensitivity. A standard response curve relating the signal-height ratio of caffeine to that of an internal standard, hexobarbital, permits quantitation of the amount of caffeine present. The method involves extraction of caffeine from plasma with chloroform, after the aqueous phase was adjusted to pH 11.5–12.0. The chloroform extract was evaporated to dryness and the sample was redissolved in carbon disulfide. Two milliliters of plasma was used and caffeine was determined at a concentration of 0.25 mcg./ml.
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