Determination of cadmium and zinc levels and oxidative status in cadmium treated developing chick embryonic liver

Abstract

In the present study Chick embryos of Bobcock strain were treated with different concentrations (0.04, 0.05and 0.06 mg/egg) of CdCl2 on the day 10 (d10), day 11 (d11), day 12(d12) of embryonic development. In this study metals like Cd, Zn were determined with ICP-OES in liver tissue of control and treated groups at different time intervals. The levels of (GSH) and activity levels of antioxidant enzymes such as glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) were measured in liver tissue after different time intervals (24 h, 48h and 72 h) of CdCl2 exposure. Significant induction was observed in GST activity in liver tissue after 24 h, 48 h and 72 h. However, the GPx, GR, SOD, CAT and GSH levels were decreased in dose and time dependent manner. In this study Cd retention in the liver increased with dose level of Cd and this inturn caused induction in the levels of Zinc in liver tissue.