DETERMINATION OF BOVINE SERUM ALBUMIN CONJUGATED DRUGS BY IMMOBILIZATION AS MICROARRAYS AND OBLIQUE-INCIDENCE REFLECTIVITY DIFFERENCE MICROSCOPY

Abstract

Human serum is a mixture of various proteins which may interact with drugs. Therefore, it is of interest to investigate the binding kinetics of pharmaceuticals with their corresponding antibodies in serum. In this article, microarrays and a label-free biosensor were used to study these interactions. Microarrays provide a high-throughput platform for characterizing biomolecular interactions, and the label-free oblique-incidence reflectivity difference biosensor avoids the drawbacks of fluorescence-based methods. The experimental results show that the binding affinities between most of the drugs and their antibodies were reduced in human serum because the bulky proteins block the access to or reduce the stability of the reaction complexes. Therefore, one should be mindful when in vitro or in vivo testing the efficiency of potential drugs and their antibodies.