Determination of Bisphenol A by High-Performance Liquid Chromatography Based on Graphene Magnetic Dispersion Solid Phase Extraction.

Abstract

Bisphenol A (BPA), as one of the environmental endocrine disruptors, is extensively existing and threatening to human health. To evaluate the environmental exposure level and protect human from the hazard of BPA, a precise and sensitive method is established. In this work, Graphene@ Fe3O4 (G@Fe3O4) is prepared by chemical coprecipitation method as magnetic dispersion solid phase extraction (MDSPE) material. The rapid and specific detection method of BPA is carried out by high-performance liquid chromatography (HPLC). Properties of G@Fe3O4 are identified by the fourier infrared spectrum and scanning electron microscopy. Conditions of solid phase extraction are optimized. Under the optimal extraction conditions, G@Fe3O4 has perfect enrichment effect on BPA. There is a good linear relationship in the range of 5.0~1000.0μg/L with the correlation coefficient of 0.9997. The detection limit is 0.1μg/L. This method is applied to water samples successfully, and recoveries of BPA are between 88.19% and 99.56% (RSDs<3.00%). G@Fe3O4 was synthesized, which was used to extract BPA in water samples before HPLC analysis, and has shown perfect extraction ability toward BPA, which indicates that the determination method of BPA by HPLC based on graphene MDSPE is faster and more precise.